Hi Ghazal, my Dear,
I hope you are doing well and feel o.k.
Is it quiet now in the Lab ? Usually this is the best time to concentrate on the project. I hope so much that you find some intellectual satisfaction with the project and not have to consider it as a hard burden and a duty to fulfill.
I prepared some text about the mapping, the markers, the mouse strains and linkage. It should somehow fit into the thesis structure (as much as I remember it was part 2 or so). Just break it apart into the several sub-headings.
And of course, if you find in the text something difficult to understand or wrong or strange, please write me back. I very much like the intellectual conversation with you and I learn a lot from your questions as well.
I will also send you something that explains the animal experiments (the older ones we did years ago and the JF1 x FVB crosses). Was Prof. T. already around ?
I think of you and your aristocratic character, that is so much different from the people here around which are very loud and miss any sense of ellegance. This is usually what one can expect at a beach,but the kids like this and therefore I have to decline.
I"m waiting to see you next week.
Take Care,my dear
T. has not been here yet. I think he chose this week on purpose. Both
you and M. are on vacation.
It is a bit quiet yes. I put aside the labwork yesterday and today to
sit and read and write. btw, the sequencing worked!! im happy. me and
claudia will maybe analyse the data later today.
it is not a burden to do this project. everything has been good so far.
I do not know how to thank you for writing so much for me. i have
written a bit about the mapping and microsatellite and QTL. but it was
not as much as yours. so thank you. you should not do this on your
vacation. just go and have fun. right now i am concentrating at the
first part with chernobyl and thyroid cancer.
take care and see you next week!
Ghazal my Dear,
Good to hear that you managed so well to continue with the project and the thesis during this week. Good luck with the sequence analysis,hope you and C. find some interesting variations between the two strains.
I attache a piece of text that describes the rational for using the JF1 and the FVB/N strain in this project. Please double-check if any of the chromosomal loci or gene names are consistent with what you wrote before. Also, in case you find any other discrepancies with the other text-pieces,please mark it in red and we will go through when I am back.
Ghazal, please don"t feel obliged to say "Thank you" for these small pieces of text. It is my very duty as a supervisor to help you with this. It is you who is doing the sophisticated and very decent experimental work day-by-day, without complaining that it is getting boring. Therefore,it is in fact me who must be extremely grateful. Don"t forget this, please.
And what is maybe even more relevant, that you always have this endless patience with me, if I come around and require that you spend the time after work with me. No MSc student has the commitment of going to the movies or to an exhibition or to the beach with its supervisor, or attend with him a concert or watch the meteor shower in the middle of the night. You could have in fact complaine with Prof. T. right from the beginning and ask for another project or another supervisor. I know I was always a bit violently when I asked you to meet me after work,and who knows how often I have disturbed your planing for the evening when you wanted to read or write something for the project. Therefore,it is the least I can do to help you to get everything together know for the thesis, it is a very small compensation for all the nice days and moments you have been with me and for having been always so patient and listened to my silly stories and telling me something from you. You could have easily send me to hell, you never did (I hope you dont wish you could have done this, but you did not wanted to risk your project).
Ghazal, you will see at the end (and everybody will agree) that a successful MSc project does not need this archetypical and hirarchical relation between student and supervisor, but it also can be done if there is a (sort of) frienship.
For me,to be honest, you are too much a mystery to call this a simple friendship.
I wish you a pleasant evening, a relaxing night, and nice dreams.
PS:How is your watch doing ? I hope that "No news are good news" and it runs precisely now, since you gave it a bit more freedom around your arm.
i think i found one mutation on the third exon.
yes u are right i could ask prof. t. for another project (and he would not give me another since i already tried in the beginning). but i enjoy it here i would not do it. u could also be a bad supervisor and not wanting to help. so i am grateful.
i send u a text with some questions. answer just if u have time. no hurry.
my watch is suprisingly doing great!its strange.
have a good day!!
Ghazal my Dear,
Congratulations for finding a sequence variation in FGF10 in one of the mouse strains..
You could check (using Ensembl and search for FGF10 in mouse,and click on the left panel on the button for variations) if this sequence alteration was already described in any other mouse strain. You should also check if it might cause an amino-acid change, in case it is still part of the coding sequence (I remember large parts of exon 3 were already 3" UTR. Maybe you find something.
Before I left, I already ordered primers to test the FGF10 transcript and expression. Could you,please write a mail to O. and ask if and where she has stored the JF1 and FVB/N RNA or cDNA from thyroid (she extracted it as part of her MSc for the expression array).
Thanks for writing that you feel o.k. with how the project developed and that you are o.k. with everything and don"t regret that you came to Munich. You gave me the nicest and most exciting summer of my life, Ghazal. I never felt so much inspiration, excitement and satisfaction to share with somebody a project and many hours after work as with you.
PS:I go through your text today and send you comments tomorrow